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Figure 4: Estimation of cellularity within the albumin-concentrated growth factor membrane 7 days after production. Cell nuclei were evidenced by fluorescence microscopy after staining with 4',6-diamidino-2-phenylindole in the left border (a), center (b), or right border of the membrane (c) (images obtained with a ×20 objective). (d) The mean nucleated cell number (n = 3) for each membrane portion. No statistical difference was found (P < 0.05) |
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