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  Indian J Med Microbiol
 

Figure 2: Metabolic activity (%) in response to Vitamin D3treatment in basal cell carcinoma, squamous cell carcinoma, and primary normal human epidermal keratinocytes after 3 h (a and a'), 48 h (b and b'), and 72 h (c and c') of Vitamin D3application. Metabolic activity was calculated by determining cell viability via the MTS assay and normalized to the relative total viable cell count, as measured by crystal violet staining and quantification. a-c provides an overview of metabolic activity in response to all applied Vitamin D3concentrations, whereas a'-c' highlights the effect of 1000 nM Vitamin D3on cellular metabolic activity. Statistical significance was defined as *P < 0.05 and **P < 0.01

Figure 2: Metabolic activity (%) in response to Vitamin D<sub>3</sub>treatment in basal cell carcinoma, squamous cell carcinoma, and primary normal human epidermal keratinocytes after 3 h (a and a'), 48 h (b and b'), and 72 h (c and c') of Vitamin D<sub>3</sub>application. Metabolic activity was calculated by determining cell viability via the MTS assay and normalized to the relative total viable cell count, as measured by crystal violet staining and quantification. a-c provides an overview of metabolic activity in response to all applied Vitamin D<sub>3</sub>concentrations, whereas a'-c' highlights the effect of 1000 nM Vitamin D<sub>3</sub>on cellular metabolic activity. Statistical significance was defined as *<i>P</i> < 0.05 and **<i>P</i> < 0.01